Biochemistry
Welkom by Universiteit Stellenbosch

Onlangse Publikasies

​Joernaal Artikels (gesubsideer)

ABIDIN MZ, SARAVANAN T, ZHANG J, TEPPER PG, STRAUSS E, POELARENDS GJ. Modular enzymatic cascade synthesis of vitamin B5and its derivatives. CHEMISTRY-A EUROPEAN JOURNAL 2018; 24:17434-17438.

AFRICANDER DJ, STORBECK K. Steroid metabolism in breast cancer: Where are we and what are we missing?. Molecular and Cellular Endocrinology 2018; 466:86-97.

ANECK-HAHN NH, VAN ZIJL MC, SWART P, TRUEBODY BE, GENTHE B, CHARMIER J, DE JAGER C. Estrogenic activity, selected plasticizers and potential health risks associated with bottled water in South Africa. JOURNAL OF WATER AND HEALTH 2018; 16(2):253-262.

BARNARD L, MOSTERT KJ, VAN OTTERLO WAL, STRAUSS E. Developing pantetheinase-resistant pantothenamide antibacterials: Structural modification impacts on PanK interaction and mode of action. ACS Infectious Diseases 2018; 4(5):736-743.

BARNARD M, QUANSON J, MOSTAGHEL E, PRETORIUS E, SNOEP JL, STORBECK K. 11-Oxygenated androgen precursors are the preferred substrates for aldo-keto reductase 1C3 (AKR1C3): Implications for castration resistant prostate cancer. Journal of Steroid Biochemistry and Molecular Biology 2018; 183:192-201.

BURGER H-M, ABEL S, GELDERBLOM WCA. Modulation of key lipid raft constituents in primary rat hepatocytes by fumonisin B1- Implications for cancer promotion in the liver. FOOD AND CHEMICAL TOXICOLOGY 2018; 115:34-41.

CHRISTENSEN C, HOFMEYR JS, ROHWER JM. Delving deeper: Relating the behaviour of a metabolic system to the properties of its components using symbolic metabolic control analysis. PLoS One 2018; 13(11):e0207983 (24 pages).

CHRISTENSEN C, HOFMEYR JS, ROHWER JM. PySCeSToolbox: a collection of metabolic pathway analysis tools. Bioinformatics 2018; 34(1):124-125.

DE BEER DM, BOTES M, CLOETE TE. The microbial community of a biofilm contact reactor for the treatment of winery wastewater. Journal of Applied Microbiology 2018; 124(2):598-610.

DU TOIT A, DE WET S, HOFMEYR JS, MULLER-NEDEBOCK KK, LOOS B. The Precision Control of Autophagic Flux and Vesicle Dynamics - A Micropattern Approach. Cells 2018; 7(8):94, 17 pages.

DU TOIT A, HOFMEYR JS, GNIADEK TJ, LOOS B. Measuring autophagosome flux. Autophagy 2018; 14(6):1060-1071.

DU TOIT T, FINKEN MJJ, HAMER HM, HEIJBOER AC, SWART AC. C11-oxy C19and C11-oxy C21steroids in neonates: UPC2-MS/MS quantification of plasma 11β-hydroxyandrostenedione, 11-ketotestosterone and 11-ketoprogesterone. STEROIDS 2018; 138:1-5.

DU TOIT T, STANDER MA, SWART AC. A high-throughput UPC2-MS/MS method for the separation and quantification of C19and C21steroids and their C11-oxy steroid metabolites in the classical, alternative, backdoor and 11OHA4 steroid pathways. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 2018; 1080:71-81.

DU TOIT T, SWART AC. Inefficient UGT-conjugation of adrenal 11ß-hydroxyandrostenedione metabolites highlights C11-oxy C19steroids as the predominant androgens in prostate cancer. Molecular and Cellular Endocrinology 2018; 461:265-276.

GUAN J, BARNARD L, CRESSON J, HOEGL A, CHANG JH, STRAUSS E, AUCLAIR K. Probing the ligand preferences of the three types of bacterial pantothenate kinase. Bioorganic and Medicinal Chemistry 2018; 26:5896-5902.

HALLING PJ, FITZPATRICK PF, RAUSHEL FM, ROHWER JM, SCHNELL S, WITTIG U, WOHLGEMUTH R, KETTNER C. An empirical analysis of enzyme function reporting for experimental reproducibility: Missing/incomplete information in published papers. BIOPHYSICAL CHEMISTRY 2018; 242:22-27.

HAMER HM, FINKEN MJJ, VAN HERWAARDEN AE, DU TOIT T, SWART AC, HEIJBOER AC. Falsely elevated plasma testosterone concentrations in neonates: Importance of LC-MS/MS measurements. CLINICAL CHEMISTRY AND LABORATORY MEDICINE 2018; 56(6):e141-e143.

HOFMEYR JS. Causation, constructors and codes. BIOSYSTEMS 2018; 164:121-127.

HOFMEYR JS. The first special issue on code biology - A bird's-eye view. BIOSYSTEMS 2018; 164:11-15.

JENKINSON C, TAYLOR AE, STORBECK K, HEWISON M. Analysis of multiple vitamin D metabolites by ultra-performance supercritical fluid chromatography-tandem mass spectrometry (UPSFC-MS/MS). JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 2018; 1087-1088:43-48.

JENKINSON C, TAYLOR AE, STORBECK K, HEWISON M. Data comparing the separation and elution of vitamin D metabolites on an ultra performance supercritical fluid chromatography tandem-mass spectrometer (UPSFC-MS/MS) compared to liquid chromatography (LC) and data presenting approaches to UPSFC method optimization. Data in Brief 2018; 20:426-435.

MAGANGANA TP, STANDER MA, MAKUNGA NP. Effect of nitrogen and phosphate on in vitro growth and metabolite profiles of Steviarebaudiana Bertoni (Asteraceae). Plant Cell, Tissue and Organ Culture 2018; 134(1):141-151.

NAGHILOO S, BELLSTEDT DU, CLAßEN-BOCKHOFF R. Nectar protection in arid-adapted flowers of Zygophyllaceae-Zygophylloideae. Perspectives in plant ecology, evolution and systematics 2018; 34:37-50.​

OYENIHI OR, KRYGSMAN A, VERHOOG NJD, DE BEER D, SAAYMAN M, MOUTON M, LOUW A. Chemoprevention of LA7-induced mammary tumor growth by SM6Met, a well-characterized CylcopiaExtact. Frontiers in Pharmacology 2018; 9:1-18.

PERKINS MS, LOUW-DU TOIT R, AFRICANDER DJ. Hormone therapy and breast cancer: emerging steroid receptor mechanisms. JOURNAL OF MOLECULAR ENDOCRINOLOGY 2018; 61(4):R133-R160.

SANTOLARIA C, VELÁZQUEZ D, STRAUSS E, ARIÑO J. Mutations at the hydrophobic core affect Hal3 trimer stability, reducing its Ppz1 inhibitory capacity but not its PPCDC moonlighting function. Scientific Reports 2018; 8:14701 (12 pages).

SCHIFFER L, ARLT W, STORBECK K. Intracrine androgen biosynthesis, metabolism and action revisited. Molecular and Cellular Endocrinology 2018; 465:4-26.

SHOKO R, MANASA J, MAPHOSA M, MBANGA J, MUDZIWAPASI R, NEMBAWARE V, SANYIKA WT, TINAGO T, CHIKWAMBI Z, MAWERE C, MATIMBA A, MUGUMBATE G, MUFANDAEDZA J, MULDER N, PATTERTON H. Strategies and opportunities for promoting bioinformatics in Zimbabwe. PLoS Computational Biology 2018; 14:1-12.

SMITH C, SWART AC. Aspalathus linearis(Rooibos) - a functional food targeting cardiovascular disease. Food & Function 2018; 9:5041-5058.

STORBECK K, GILLIGAN L, JENKINSON C, BARANOWSKI ES, QUANSON J, ARLT W, TAYLOR AE. The utility of ultra-high performance supercritical fluid chromatography–tandem mass spectrometry (UHPSFC-MS/MS) for clinically relevant steroid analysis. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 2018; 1085:36-41.

SWAINSTON N, BAICI A, BAKKER BM, CORNISH-BOWDEN A, FITZPATRICK PF, HALLING PJ, LEYH TS, O'DONOVAN C, RAUSHEL FM, RESCHEL U, ROHWER JM, SCHNELL S, SCHOMBURG D, TIPTON K, TSAI M-D, WESTERHOFF HV, WITTIG U, WOHLGEMUTH R, KETTNER C. STRENDA DB: enabling the validation and sharing of enzyme kinetics data. FEBS Journal 2018; 285:2193-2204.

TJHIN ET, SPRY C, SEWELL AL, HOEGL A, BARNARD L, SEXTON AE, SIDDIQUI G, HOWIESON VM, MAIER AG, CREEK DJ, STRAUSS E, MARQUEZ R, AUCLAIR K, SALIBA KJ. Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues. PLoS Pathogens 2018; 14(4):e1006918 (30 pages).

VAN ROOYEN D, GENT R, BARNARD L, SWART AC. The in vitro metabolism of 11β-hydroxyprogesterone and 11-ketoprogesterone to 11-ketodihydrotestosterone in the backdoor pathway. Journal of Steroid Biochemistry and Molecular Biology 2018; 178:203-212.

VENTER PB, MULLER MM, VESTNER J, STANDER MA, TREDOUX AGJ, PASCH H, DE VILLIERS AJ. Comprehensive three-dimensional LC x LC × ion mobility spectrometry separation combined with high-resolution MS for the analysis of complex samples. Analytical Chemistry 2018; 90(19):11643-11650.

WEGER M, DIOTEL N, WEGER BD, BEIL T, ZAUCKER A, EACHUS H, OAKES JA, DO REGO JL, STORBECK K, GUT P, STRÄHLE U, RASTEGAR S, MÜLLER F, KRONE N. Expression and activity profiling of the steroidogenic enzymes of glucocorticoid biosynthesis and the fdx1 co-factors in zebrafish. JOURNAL OF NEUROENDOCRINOLOGY 2018; 30:e12586 (15 pages).

WENZEL M, RAUTENBACH M, VOSLOO JA, SIERSMA T, AISENBREY CHM, ZAITSEVA E, LAUBSCHER WE, VAN RENSBURG W, BEHRENDS JC, BECHINGER B, HAMOEN LW. The multifaceted antibacterial mechanisms of the pioneering peptide antibiotics Tyrocidine and Gramicidin S. mBio 2018; 9(5):e00802-18 (20 pages).

WILKINSON L, VERHOOG NJD, LOUW A. Disease- and treatment-associated acquired glucocorticoid resistance. Endocrine Connections 2018; 7:R328-R349.

WILKINSON L, VERHOOG NJD, LOUW A. Novel role for receptor dimerization in post-translational processing and turnover of the GRα.Scientific Reports 2018; 8:14266 (17 pages).

Joernaal Artikels (nie-gesubsideer)​

GIESSNER C, MILLET V, MOSTERT KJ, GENSOLLEN T, VU MANH T-P, GARIBAL M, DIEME B, ATTAF-BOUABDALLAH N, CHASSON L, BROUILLY N, LAPRIE C, LESLUYES T, BLAY JY, SHINTU L, MARTIN JC, STRAUSS E, GALLAND F, NAQUET P. Vnn1 pantetheinase limits the Warburg effect and sarcoma growth by rescuing mitochondrial activity. Life Science Alliance 2018; 1(4):e201800073 (16 pages).​

Paten​​​te

LOOS B, HOFMEYR JS, PEROLD WJ. Method and device for determining autophagic flux. Patent No. P3464ZA00SC, South Africa 2018.

Doktoral voltooi 

DU TOIT T. The metabolism of 11β-hydroxyandrostenedione by steroidogenic enzymes yields metabolites contributing to the androgen pool in prostate cancer. PhD, 2018. 223 pp. Promotor: SWART AC.

PERKINS MS. Investigating the mechanism of action of hormones used in hormone replacement therapy via estrogen receptor subtypes and the influence of the progesterone receptor. PhD, 2018. 243 pp. Promotor: AFRICANDER DJ. Medepromotor: LOUW-DU TOIT R.

ROOS WG. An assessment of the diversity and pathogenicity of Potato Leaf Roll Virus in South Africa. PhD, 2018. 215 pp. Promotor: BELLSTEDT DU.

TAIT T. Strategies for the detection of endocrine disrupting compounds utilizing recombinant oestrogen receptor ligand-binding domains and high specificity monoclonal antibodies. PhD, 2018. 202 pp. Promotor: SWART P. Medepromotor: SWART AC.

WILKINSON L. Homologous down-regulation of the glucocorticoid receptor is influenced by the dimerization state of the receptor. PhD, 2018. 266 pp. Promotor: LOUW A. Medepromotor: VERHOOG NJD.

Meesters voltooi

ANDREWS CJ. Cyclopia extracts and sex hormone binding globulin (SHBG). MSc, 2018. 153 pp. Studieleier: LOUW A. Medestudieleier: VERHOOG NJD.

BERGE S. Production and characterisation of analogues of the antimicrobial tyrocidine peptides with modified aromatic amino acid residues. MSc, 2018. 166 pp. Studieleier: RAUTENBACH M.

BOUWER W. Investigation and Characterisation of Dephospho-Coenzyme A kinase: A Potential Drug Target. MSc, 2018. 87 pp. Studieleier: DE VILLIERS M. Medestudieleier: STRAUSS E.

CABRAL AA. Crosstalk between the androgen receptor and progesterone receptor isoforms in breast and prostate cancer. MSc, 2018. 126 pp. Studieleier: AFRICANDER DJ. Medestudieleier: STORBECK K.

CLAASSEN H. Investigating the parts to understand the whole: Contribution of phenolic compounds to the desired estrogenic activity of SM6Met fractions from Cyclopia subternata . MSc, 2018. 165 pp. Studieleier: LOUW A. Medestudieleier: VISSER JAK.

GENT R. An investigation into the catalytic activity of 11β-hydroxysteroid dehydrogenase towards C11-oxy C19 and C11-oxy C21 steroids. MSc, 2018. 165 pp. Studieleier: SWART AC.

JOHANNES ID. The in vitro inhibition of adrenal steroidogenic enzymes and modulation of adrenal hormone production by Sceletium tortuosum . MSc, 2018. 90 pp. Studieleier: SWART AC. Medestudieleier: SMITH C.

RAS TA. Bioinformatic characterisation of genes associated with coenzyme A biosynthesis in mycoplasmas and expression and isolation of dephospho-coenzyme A kinase from Mycoplasma sp. Ms02. MSc, 2018. 148 pp. Studieleier: BOTES A. Medestudieleier: STRAUSS E.

SWANEPOEL CJ. A systematic investigation into the quantitative effect of pH changes on the upper glycolytic enzymes of Escherichia coli and Saccharomyces cerevisiae. MSc, 2018. 120 pp. Studieleier: ROHWER JM.

VAN DYK L. Combinatorial treatments of tamoxifen with SM6Met, a selective estrogen receptor subtype modulator (SERSM), from Cyclopia subternata are superior to current endocrine treatments in breast cancer cell models. MSc, 2018. 148 pp. Studieleier: LOUW A. Medestudieleier: VERHOOG NJD.​​​