Biochemistry
Welkom by Universiteit Stellenbosch

Onlangse Publikasies

Joernaal Artikels ​​(gesubsideer)

AFRICANDER DJ, LOUW-DU TOIT R. Progestins in menopausal hormone therapy and breast cancer risk: The debate continues. Current Opinion in Endocrine and Metabolic Research 2020; 15:24-30.

BARNARD L, NIKOLAOU N, LOUW C, SCHIFFER L, GIBSON H, GILLIGAN L, GANGITANO E, SNOEP JL, ARLT W, TOMLINSON JW, STORBECK K. The A-ring reduction of 11-ketotestosterone is efficiently catalysed by AKR1D1 and SRD5A2 but not SRD5A1. Journal of Steroid Biochemistry and Molecular Biology 2020; 202:art no. 105724, 10 pages.

BARNARD M, MOSTAGHEL E, AUCHUS RJ, STORBECK K. The role of adrenal derived androgens in castration resistant prostate cancer. Journal of Steroid Biochemistry and Molecular Biology 2020; 197:art no. 105506, 14 pages.

BELLSTEDT DU. The building blocks and origins of life. HTS Theological Studies/ Teologiese Studies 2020; 76(1):a6054, 7 pages.
BUTMAN HS, KOTZÉ TJ, DOWD CS, STRAUSS E. Vitamin in the crosshairs: Targeting Pantothenate and Coenzyme A biosynthesis for new antituberculosis agents. Frontiers in Cellular and Infection Microbiology 2020; 10:art no. 605662, 33 pages.

DU TOIT T, SWART AC. The 11β-hydroxyandrostenedione pathway and C11-oxy C21 backdoor pathway are active in benign prostatic hyperplasia yielding 11ketotestosterone and 11keto-progesterone. Journal of Steroid Biochemistry and Molecular Biology 2020; 196:art no. 105497, 14 pages.

DU TOIT T, VAN ROOYEN D, STANDER MA, ATKIN SL, SWART AC. Analysis of 52 C19 and C21 steroids by UPC2-MS/MS: Characterising the C11-oxy steroid metabolome in serum. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 2020; 1152:art no. 122243, 20 pages.

ENFIELD K, CARTWRIGHT M, LOUW-DU TOIT R, AVENANT C, AFRICANDER DJ, HAPGOOD JP. Characterisation of progestins used in hormonal contraception and progesterone via the progesterone receptor. Biochemical and Biophysical Research Communications 2020; 533:879-885.

GURAN T, KARA C, YILDIZ M, BITKIN EC, HAKLAR G, LIN J-C, KESKIN M, BARNARD L, ANIK A, CATLI G, STORBECK K, ET AL ET AL. Revisiting Classical 3β-hydroxysteroid Dehydrogenase 2 Deficiency: Lessons from 31 Pediatric Cases. Journal of Clinical Endocrinology and Metabolism 2020; 105(4):e1718-e1728.

HOFMEYR JS. Kinetic modelling of compartmentalised reaction networks. BIOSYSTEMS 2020; 197:art no. 104203, 17 pages.

JOKONYA S, LANGLAIS MAP, LESHABANE M, READER PW, VOSLOO JA, PFUKWA R, COERTZEN D, BIRKHOLTZ L, RAUTENBACH M, KLUMPERMAN L. Poly(N-vinylpyrrolidone) antimalaria conjugates of membrane-disruptive peptides. Biomacromolecules 2020; 21(12):5053-5066.

LEBEPE CM, MATAMBANADZO PR, MAKHOBA XH, ACHILONU I, ZININGA T, SHONHAI A. Comparative Characterization of Plasmodium falciparum Hsp70-1 Relative to E. coli DnaK Reveals the Functional Specificity of the Parasite Chaperone. Biomolecules 2020; 10:art no. 856, 21 pages.

LI N, OAKES JA, STORBECK K, CUNLIFFE VT, KRONE NP. The P450 side-chain cleavage enzyme Cyp11a2 facilitates steroidogenesis in zebrafish. JOURNAL OF ENDOCRINOLOGY 2020; 244(2):309-321.

LOOS B, KLIONSKY DJ, DU TOIT A, HOFMEYR JS. On the relevance of precision autophagy flux control in vivo - Points of departure for clinical translation. Autophagy 2020; 16(4):750-762.

LOUW-DU TOIT R, HAPGOOD JP, AFRICANDER DJ. A direct comparison of the transcriptional activities of progestins used in contraception and menopausal hormone therapy via the mineralocorticoid receptor. Biochemical and Biophysical Research Communications 2020; 526:466-471.

LÜBCKER N, BLOEM LM, DU TOIT T, SWART P, DE BRUYN PJN, SWART AC, MILLAR RP. What's in a whisker? High-throughput analysis of twenty-eight C19 and C21 steroids in mammalian whiskers by ultra-performance convergence chromatography-tandem mass spectrometry. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 2020; 1141:art no. 122028, 10 pages.

MAKUMIRE S, ZININGA T, VAHOKOSKI J, KURSULA I, SHONHAI A. Biophysical analysis of Plasmodium falciparum Hsp70-Hsp90 organising protein (PfHop) reveals a monomer that is characterised by folded segments connected by flexible linkers. PLoS One 2020; 15(4):e0226657, 13 pages.

MARCHI D, SANTHAKUMAR K, MARKHAM E, LI N, STORBECK K, KRONE NP, CUNLIFFE VT, VAN EEDEN FJM. Bidirectional crosstalk between Hypoxia-Inducible Factor and glucocorticoid signaling in zebrafish larvae. PLoS Genetics 2020; 16(5):e1008757, 28 pages.

MASIKE K, DE VILLIERS AJ, HOFFMAN EW, BRAND DJ, CAUSON T, STANDER MA. Detailed phenolic characterization of Protea pure and hybrid cultivars by liquid chromatography-ion mobility-high resolution mass spectrometry (LC-IM-HR-MS). Journal of Agricultural and Food Chemistry 2020; 68(2):485-502.

MASIKE K, DE VILLIERS AJ, HOFFMAN EW, STANDER MA. Application of metabolomics tools to determine possible biomarker metabolites linked to leaf blackening in Protea. Journal of Agricultural and Food Chemistry 2020; 68(45):12595-12605.

NAGHILOO S, BELLSTEDT DU, CLASSEN-BOCKHOFF R. Pollination biology in Roepera (Zygophyllaceae): How flower structure and shape influence foraging activity. PLANT SPECIES BIOLOGY 2020; 35:72-80.

NAGY B, WATTERS BR, VAN DER MERWE PDW, COTTERILL FPD, BELLSTEDT DU. Review of the Nothobranchius ugandensis species group from the inland plateau of eastern Africa with descriptions of six new species (Teleostei: Nothobranchiidae). ICHTHYOLOGICAL EXPLORATION OF FRESHWATERS 2020; 30(1):21-73.

NEVELING D, AHIRE J, LAUBSCHER WE, RAUTENBACH M, DICKS LMT. Correction to: Genetic and Phenotypic Characteristics of a Multi strain Probiotic for Broilers. Current Microbiology 2020; 77:1955-1956.

NEVELING D, AHIRE J, LAUBSCHER WE, RAUTENBACH M, DICKS LMT. Genetic and Phenotypic Characteristics of a Multi strain Probiotic for Broilers. Current Microbiology 2020; 77:369-387.

NGUGI G, LE PÉCHON T, MARTOS F, PAILLER T, BELLSTEDT DU, BYTEBIER B. Phylogenetic relationships amongst the African genera of subtribe Orchidinae s.l. (Orchidaceae; Orchideae): Implications for subtribal and generic delimitations. Molecular Phylogenetics and Evolution 2020; 153:art no. 106946, 8 pages.

OAKES JA, BARNARD L, STORBECK K, CUNLIFFE VT, KRONE NP. 11β-Hydroxylase loss disrupts steroidogenesis and reproductive function in zebrafish. JOURNAL OF ENDOCRINOLOGY 2020; 247(2):197-212.

PADAYACHEE L, ROHWER JM, PILLAY CS. The thioredoxin redox potential and redox charge are surrogate measures for flux in the thioredoxin system. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 2020; 680:art no. 108231, 12 pages.

PERRECA E, ROHWER JM, GONZÁLEZ-CABANELAS D, LORETO F, SCHMIDT A, GERSHENZON J, WRIGHT LP. Effect of drought on the Methylerythritol 4-Phosphate (MEP) pathway in the Isoprene emitting conifer Picea glauca. Frontiers in Plant Science 2020; 11:art no. 546295, 14 pages.

SAASA V, LEMMER Y, MALWELA T, AKANDE A, BEUKES M, MWAKIKUNGA B. Effect of varying ethanol and water compositions on the acetone sensing properties of WO3 for application in diabetes mellitus monitoring. Materials Research Express 2020; 7:art no. 035905, 13 pages.

SAASA V, MALWELA T, LEMMER Y, BEUKES M, MWAKIKUNGA B. The hierarchical nanostructured Co-doped WO3/carbon and their improved acetone sensing performance. MATERIALS SCIENCE IN SEMICONDUCTOR PROCESSING 2020; 117:art no. 105157, 9 pages.

SHARMA N, AHALAWAT N, SANDHU P, STRAUSS E, MONDAL J, ANAND R. Role of allosteric switches and adaptor domains in long-distance cross-talk and transient tunnel formation. Science Advances 2020; 6:eaay7919, 11 pages.

SHEN L, KOHLHAAS M, ENOKI J, MEIER R, SCHÖNENBERGER B, WOHLGEMUTH R, KOURIST R, NIEMEYER F, VAN NIEKERK DD, BRÄSEN C, NIEMEYER J, SNOEP JL, SIEBERS B. A combined experimental and modelling approach for the Weimberg pathway optimisation. Nature Communications 2020; 11:art no. 1098, 13 pages.

SPERGSER J, BOTES A, NEL TI, RUPPITSCH W, LEPUSCHITZ S, LANGER S, RIES S, DINHOPL N, SZOSTAK M, LONCARIC I, BUSSE H-J. Mycoplasma nasistruthionis sp. nov. and Mycoplasma struthionis sp. nov. isolated from ostriches with respiratory disease. SYSTEMATIC AND APPLIED MICROBIOLOGY 2020; 43:art no. 126047, 7 pages.

SPRY C, BARNARD L, KOK M, POWELL AK, MAHESH D, TJHIN ET, SALIBA KJ, STRAUSS E, DE VILLIERS M. Toward a Stable and Potent Coenzyme A Targeting Antiplasmodial Agent: Structure−Activity Relationship Studies of N Phenethyl-α-methyl-pantothenamide. ACS Infectious Diseases 2020; 6:1844-1854.

STORBECK K, MCNAMARA KM. The importance of mass spectrometry in unravelling steroid action in breast cancer. Current Opinion in Endocrine and Metabolic Research 2020; 15:57-62.

SWART AC, DU TOIT T, GOURGARI E, KIDD M, KEIL M, FAUCZ FR, STRATAKIS C. Steroid hormone analysis of adolescents and young women with polycystic ovarian syndrome and adrenocortical dysfunction using UPC²-MS/MS. PEDIATRIC RESEARCH 2020; doi.org/10.1038/s41390-020-0870-1:0.

TSHALIBE RS, RHEEDER JP, ALBERTS JF, TALJAARD-KRUGELL C, GELDERBLOM WCA, SHEPHARD GS, LOMBARD MJ, BURGER H-M. Multi-mycotoxin exposure of children (0-24 months) in rural maize-subsistence farming areas of the Eastern Cape Province, South Africa. World Mycotoxin Journal 2020; 13(3):401-410.

VAN ROOYEN D, YADAV R, SCOTT EE, SWART AC. CYP17A1 exhibits 17αhydroxylase/17,20-lyase activity towards 11β-hydroxyprogesterone and 11-ketoprogesterone metabolites in the C11-oxy backdoor pathway. Journal of Steroid Biochemistry and Molecular Biology 2020; 199:art no. 105614, 12 pages.

VIVIERS CGA, DU TOIT A, PEROLD WJ, LOOS B, HOFMEYR JH. A resistive biosensor for the detection of LC3 Protein in Autophagy. IEEE SENSORS JOURNAL 2020; 20(10):5119-5129.

VOSLOO JA, RAUTENBACH M. Following tyrothricin peptide production by Brevibacillus parabrevis with electrospray mass spectrometry. BIOCHIMIE 2020; 179:101-112.

WATTERS BR, NAGY B, VAN DER MERWE PDW, COTTERILL FPD, BELLSTEDT DU. Redescription of the seasonal killifish species Nothobranchius ocellatus and description of a related new species Nothobranchius matanduensis, from eastern Tanzania (Teleostei: Nothobranchiidae). ICHTHYOLOGICAL EXPLORATION OF FRESHWATERS 2020; 30(2):151-178.

WATTERS BR, NAGY B, VAN DER MERWE PDW, COTTERILL FPD, BELLSTEDT DU. Review of the Nothobranchius taeniopygus species group from central and western Tanzania with descriptions of five new species and redescription of Nothobranchius taeniopygus (Teleostei: Nothobranchiidae). ICHTHYOLOGICAL EXPLORATION OF FRESHWATERS 2020; 29(3):239-278.


Joernaal Artikels (nie-gesubsideer)

DEPFENHART M, LEMPERLE G, MEYER M, RAUTENBACH M, BERTOSSI D, DE VILLIERS D. A SARS-CoV-2 Prophylactic and Treatment; A Counter Argument Against The Sole Use of Chloroquine. American Journal of Biomedical Science & Research 2020; 8(4):248-251.


Hoofstukke in Boeke​

DU TOIT A, LOOS B, HOFMEYR JS. Supply and demand analysis of Autophagy. In: Nagrath D (ed.) Metabolic Flux Analysis in Eukaryotic Cells, Springer Science & Business Media, New York, USA, 2020: 345-358.


Patente

KLUMPERMAN L, READER PW, RAUTENBACH M. Conjugate for treating malaria. Patent No. US 10,479,854, USA 2019.

RAUTENBACH M, VAN RENSBURG W. Method for preventing or treating microbial growth on a manufactured product. Patent No. AU2015270120, Australia 2019.

RAUTENBACH M, VAN RENSBURG W. Method for preventing or treating microbial growth on a manufactured product. Patent No. US 10,512,271, USA 2019.


Doktorale voltooi

BARNARD L. An investigation into the biosynthesis, metabolism and activity of 11-Oxygenated estrogens. PhD, 2020. 175 pp. Promotor: STORBECK K. Medepromotor: Arlt W.

BARNARD M. An investigation into the role of 11-oxygenated androgens in castration resistant prostate cancer. PhD, 2020. 217 pp. Promotor: STORBECK K. Medepromotor: AFRICANDER DJ.

LOUW C. Computational modelling of steroid hormone biosynthesis and metabolism. PhD, 2020. 152 pp. Promotor: SNOEP JL. Medepromotor: VAN NIEKERK DD.

MAREE JP. Identification and genomic distribution of select combinatorial post-translational modification patterns of Trypanosoma brucei histones. PhD, 2020. 166 pp. Promotor: PATTERTON H. Medepromotor: LOUW A.

MOGWERA KSP. Membrane permeability and transport studies of Coenzyme A, its precursors and antimetabolites. PhD, 2020. 117 pp. Promotor: STRAUSS E. Medepromotor: DE VILLIERS M.

VAN DYK JB. Mathematical modelling of glycolysis in skeletal muscle cells. PhD, 2020. 168 pp. Promotor: SNOEP JL. Medepromotor: VAN NIEKERK DD.

VAN RENSBURG W. The tyrocidines in the creation of antimicrobial cellulose and sterilising materials. PhD, 2020. 203 pp. Promotor: RAUTENBACH M.

VAN ROOYEN D. An investigation into the biosynthesis and metabolism of adrenal C11-oxy steroids. PhD, 2020. 287 pp. Promotor: SWART AC. Medepromotor: VERHOOG NJD.


Meesters voltooi

BADENHORST M. Investigating the dynamics of hydrogen peroxide metabolism in the peroxiredoxin system of Saccharomyces cerevisiae. MSc Biochemie, 2020. 103 pp. Studieleier: ROHWER JM. Medestudieleier: Pillay CS.

BURGER A. An in vitro investigation into the effects of 11-oxygenated androgens in muscle. MSc Biochemie, 2020. 66 pp. Studieleier: STORBECK K. Medestudieleier: Kohn T, SNOEP JL.

HEITMANN S. Characterising the gut microbiome of ostrich chicks reared under intensive conditions. MSc Biochemie, 2020. 188 pp. Studieleier: BOTES A.

MEYER JP. Whole-body modelling of glucose and lactate dynamics in Plasmodium falciparum malaria. MSc Biochemie, 2020. 166 pp. Studieleier: VAN NIEKERK DD. Medestudieleier: SNOEP JL.

OCTOBER FM. Effect of yeasts and oenological parameters on acetaldehyde production during alcoholic fermentation of South African grape must. MSc Biochemie, 2020. 59 pp. Studieleier: Van Jaarsveld FP. Medestudieleier: DU TOIT WJ, ROHWER JM.

SHAW K. Quantification of the insulin response in rat L6 skeletal muscle cells. MSc Biochemie, 2020. 59 pp. Studieleier: SNOEP JL. Medestudieleier: VAN NIEKERK DD.

SPEELMAN TS. The effect of acute phase proteins on hepatic insulin signalling. MSc Biochemie, 2020. 133 pp. Studieleier: VERHOOG NJD. Medestudieleier: LOUW A.

WISSING J. An investigation into the effects of macromolecular crowding on the kinetics of upper glycolytic enzymes in Saccharomyces cerevisiae. MSc Biochemie, 2020. 55 pp. Studieleier: ROHWER JM.​​