Institute for Plant Biotechnology
Welkom by Universiteit Stellenbosch

Publikasie Argief

2010

  • Basson CE, Groenewald JH, Kossmann J, Cronjé C, Bauer R: Sugar and acid-related quality attributes and enzyme activities in strawberry fruits: invertase is the main sucrose hydrolysing enzyme. Food chemistry, 2010, 121: 1156 – 1162
  • Bastian R, Dawe A, Meier S, Ludidi N, Bajic VB, Gehring C: Gibberelic acid and cGMP-dependent transcriptional regulation in Arabidopsis Thaliana. Plant Signaling & Behavior, 2010, 5: 1-9
  • Keyster M, Klein A, Ludidi N: Endogenous NO levels regulate nodule functioning. Potential role of CGMP in nodule functioning? Plant Signalling & Behaviour, 2010, 512: 1-3
  • Kiggundu A, Muchwezi J, van der Vyver C, Viljoen A, Vorster J, Schlueter U, Kunert K, Michaud D: Deleterious effects of plant cystatins against the banana weevil Cosmopolites sordidus. Archives of insect biochemistry and physiology, 2010, 73: 87-105
  • Koetting O, Kossmann J, Zeeman SC, Lloyd JR: Regulation of starch metabolism: the age of enlightenment? Current opinion in Plant Biology, 2010, 13: 1-9
  • Leach J, Keyster M, Du Plessis M, Ludidi N: Nitric oxide synthase activity is required for developement of functional nodules in soybean. Journal of Plant Physiology, 2010, 167(18): 1584-1591
  • Lütken H, Lloyd JR, Mikkel AG, Baunsgaard L, Holst Laursen K, Haldrup A, Kossmann J, Blennow A: Repression of both isoforms of disproportionating enzyme leads to higher malto-oligosacchardie content and reduced growth in potato. Planta, 2010, 232(5): 1127-1139
  • Rossouw D, Kossmann J, Botha FC, Groenewald JH: Reduced neutral invertase activity in the culm tissues of transgenic sugarcane plants results in a decrease in respiration and sucrose cycling and an increase in the sucrose to hexose ration. Functional Plant Biology, 2010, 37: 22-31
  • Van der Merwe MJ, Groenewald JH, Stitt M, Kossmann J, Botha FC: Downregulation of pyrophosphate: d-fructose-6-phosphate 1-phosphotransferase activity in sugarcane culms enhances sucrose accumulation due to elevated hexose –phophate levels. Planta, 2010, 231: 595-608
  • Van der Vyver C: Genetic transformation of the euploid Saccharum officinarum via direct and indirect emryogenesis. Sugar Tech, 2010, 12(1): 21-25
  • Van der Vyver C., Stander C., Kossmann J. And Groenewald H. Establishment of alternative selection systems for transgenic sugarcane callus. In: Aspects of Applied Biology 96, 2010, Agriculture: Africa's "engine for growth" – Plant science & biotechnology hold the key, pp. 291-295.
  • Zeeman SC, Kossmann J, Smith AM: Starch: its metabolism, evolution, and biotechnological modification in plants. Annual Review of Plant Biology, 2010, 61: 209-234
  • Bauer R, du Toit M, Kossmann J: Influence of environmental parameters on production of the acrolein precursor 3-hydroxypropionaldehyde by Lactobacillus reuteri DSMZ 20016 and its accumulation by wine lactobacilli. International Journal of Food Microbiology, 2010, 137(1): 28-31

 

2009

  • Bauer R, Bekker J P, van Wyk N, du Toit C, Dicks L M T, Kossmann J: Exopolysaccharide production by lactose-hydrolyzing bacteria isolated from traditionally fermented milk. International Journal of Food Mircobiology, 2009, 131: 260-264
  • Esterhuyse MM, Venter M, Veldhoen N, Helbing CC, van Wyk JH: Characterization of vtg-1 mRNA expression during ontogeny in Oreochromis mossambicus (PETERS). Journal of Steroid Biochemistry & Molecular Biology, 2009, 117: 42-49
  • Nashilevitz S, Melamed-Bessudo C, Aharoni A, Kossmann J, Wolf S, Levy AA: The legwd mutant uncovers the role of starch phophorylation in pollen development and germination in tomato. The Plant journal, 2009, 57:1-13 

 

2008

  • Bauer R, Nieuwoudt H, Bauer FF, Kossmann JM, Koch KR, Esbensen KH: FTIR spectroscopy for grape and wine analysis. Analytical Chemistry, 2008, 80: 1371-1379
  • Burger BV, Viviers MZ, Bekker JPI, le Roux M, Fourie WB, Weibchen G: Chemical characterization of territorial marking fluid of male bengal tiger, Paanthera tigris. Journal of Chemical Ecology, 2008, 34(5): 659-671
  • Cullis CA, Voster BJ, Van der Yvver C., Kunert KJ. Transfer of genetic material between the chloroplast and nucleus: how is it related to stress in plants? Annals of Botany, 2008, 103(4): 625-635
  • Ferreira SJ, Kossmann J, Lloyd JR, Groenewald JH: The reduction of starch accumulation in transgenic sugarcane cell suspension culture lines. Biotechnology journal, 2008, 3(1):1398-1406
  • Martins CM, Beyene G, Hofs JL, Krueger K, Van der Vyver C, Schlueter U, Kunert KJ: Effect of water-deficit stress on cotton plants expressing the Bacillus thuringiensis toxin. Annals of Applied Biology, 2008, 152: 255-262
  • Sun H, Scharff-Poulsen AM, Gu H, Jakobsen I, Kossmann JM, Frommer WB, Almdal K: Phosphate sensing by fluorescent reporter proteins embedded in polyacrylamide nanoparticles. ACSNano, 2008, 2(1): 19-24
  • White EJ, Venter M, Hiten NF, Burger JT: Modified cetyltrimethlammonium bromide method improves robustness and versatility: the benchmark for plant RNA extraction. Biotechnology Journal, 2008, 3: 1424-1428
  • Kleinert A, Venter M, Kossmann J, Valentine A J: Role of Pepc isoforms in Phosphate-deficient nodules. In: Biological Nitrogen Fixation. Towards Poverty Alleviation through Sustainable Agriculture. Springer Science. 2008

 

2007

  • Groenewald J-H & Botha FC: Down-regulation of pyrophosphate: fructose 6-phosphate 1-phosphotransferase (PFP) activity in sugarcane enhances sucrose accumulation in immature internodes. Transgenic Research, 2007, 17(1):85-92
  • Groenewald J-H & Botha FC: Molecular and kinetic characterisation of sugarcane pyrophosphate: fructose-6-phosphate 1-phosphotransferase (PFP) and its possible role in the sucrose accumulation phenotype. Functional Plant Biology, 2007, 34: 517-525
  • Ludidi NN, Pellny TK, Kiddle G, Dutilleul C, Groten K, VAN Heerden PD, Dutt S, Powers SJ, Romer P, Foyer CH: Genetic variation in pea (Pisum sativum L.) demonstrates the importance of root but not shoot C/N ratios in the control of plant morphology and reveals a unique  relationship between shoot length and nodulation intensity. Plant, Cell & Environment, 2007, 30:1256-1268
  • Rossouw D, Bosch S, Kossmann JM, Botha FC & Groenewald J-H: Down-regulation of neutral invertase activity in sugarcane cell suspension cultures leads to increased sucrose accumulation. Functional Plant Biology, 2007, 34: 490-498
  • Venter, M: Synthetic promoters: genetic control through cis engineering. Trends in Plant Science, 2007, 12(3): 118 -124   
  • Taylor, NJ, PN Hills and J van Staden: Cell division versus cell elongation: the control of radicle elongation during thermoinhibition of Tagetes minuta achenes. Journal of Plant Physiology, 2007, 164: 1612-1625

 

2006

  • Venter M, Groenewald J-H & Botha FC: Sequence analysis and transcriptional profiling of two vacuolar H+-pyrophosphatase isoforms in Vitis vinifera. Journal of Plant Research, 2006, 119: 469–478

 

2005

  • Botha FC: Biotechnology continues to move closer to wide-scale application in the industry: the IVth Molecular Biology Workshop, held at CIRAD, Montpellier, France. Proc Int Soc Sug Cane Technol. 2005, 25: 585-591
  • Gouws LM, Osmond BC, Schurr U, Walter A: Distinctive diel growth cycles in leaves and cladodes of CAM plants: differences from C3 plants and putative interactions with substrate availability, turgor and cytoplasmic pH. Functional Plant Biology, 2005, (32): 421–428
  • Schäfer WE, Rohwer JM and Botha FC: Partial purification and characterization of sucrose synthase in sugarcane. J Plant Phys, 2005, 162: 11-20
  • Watt DA, McCormick AJ, Govender C, Carson DL, Cramer MD, Huckett BI and Botha FC: Increasing the utility of genomics in unraveling sucrose accumulation. Field Crops Res, 2005, 92: 149-158

 

2004

  • Bosch S, Grof CPL and Botha FC: Expression of neutral invertase in sugarcane. Plant Sci, 2004, 166: 1125-1133
  • Hoepfner SW and Botha FC: Purification and characterization of fructokinase from the culm of sugarcane. Plant Sci, 2004, 167: 646-654
  • Schäfer WE, Rohwer JM and Botha FC: Protein-level expression and localization of sucrose synthase in the sugarcane culm. Physiologia Plantarum, 2004, 121: 187-195
  • Schäfer WE, Rohwer JM and Botha FC: A kinetic study of sugarcane sucrose synthase. Eur J Biochem, 2004, 271: 3971-3977

 

2003

  • Bosch S, Rohwer JM and Botha FC: The sugarcane metabolome. Proc S Afr Sug Technol Ass, 2003, 77: 129-133
  • Hoepfner SW and Botha FC: Expression of fructokinase isoforms in the sugarcane culm. Plant Physiology and Biochemistry, 2003, 41: 741-747
  • Van der Merwe J, Groenewald S and Botha FC: Isolation and evaluation of a developmentally regulated sugarcane promoter. Proc S Afr Sug Technol Ass, 2003, 77: 146-149
  • Venter M and Botha FC: Promoter analysis and transcription profiling: Integration of genetic data enhances understanding of gene expression. Physiologia Plantarum, 2003, 119: 1-10

 

2002

  • Bindon KA and Botha FC: Carbon allocation to the insoluble fraction, respiration and triose-phosphate cycling in the sugarcane culm. Physiologia Plantarum, 2002, 116: 12-19
  • Carson DL and FC Botha: Genes expressed in sugarcane maturing internodal tissue. Plant Cell Reports, 2002, 20: 1075-1081
  • Carson DL, Huckett BI and Botha FC: Sugarcane ESTs differentially expressed in immature and maturing internodal tissue. Plant Sci, 2002, 162: 289-300
  • Carson DL, Huckett BI and Botha FC: Differential gene expression in sugarcane leaf and internodal tissues of varying maturity. S A J Bot, 2002, 68: 434
  • Carson DL, Huckett BI and Botha FC: Gene discovery and expression analysis in sugarcane – the story so far. Proc S Afr Sug Technol Ass, 2002, 76: 224-227
  • Turner W and Botha FC: Purification and kinetic properties of UDP-glucose dehydrogenase from sugarcane. Arch Biochem Biophys, 2002, 407: 209-216

 

2001

  • Bindon KA and Botha FC: Tissue disks as an experimental system for metabolic flux analysis in the sugarcane culm. S A J Botany, 2001, 67: 244-249
  • Botha FC: Book review on the history of plant biotechnology. Nature, 2001, 414: 397-398
  • Botha FC and Rohwer J: Kinetic modelling of sucrose metabolism: a powerful predictive tool for genetic manipulation of sugar content in sugarcane. Proc S Afr Sug Technol Ass, 2001, 75: 99-100
  • Botha FC, Sawyer BJB and Birch RG: Sucrose metabolism in the culm of transgenic sugarcane with reduced soluble acid invertase activity. Proc Int Soc Sug Cane Technol, 2001, 24: 588-591
  • Carson DL, Huckett BI and Botha FC: Genomics Research at SASEX: Perspectives from a small-scale programme. Proc Int Soc Sug Cane Technol, 2001, 24: 539-541
  • Groenewald J-H and Botha FC: Manipulating sucrose metabolism with a single enzyme: pyrophosphate-dependent phosphofructokinase (PFP). Proc S Afr Sug Technol Ass, 2001, 75: 101-103
  • Groenewald J-H and Botha FC: Down regulating pyrophosphate-dependent phosphofructokinase (PFP) in sugarcane. Proc Int Soc Sug Cane Technol, 2001, 24: 592-594
  • Rohwer JM and Botha FC: Analysis of sucrose accumulation in the sugarcane culm on the basis of in vitro kinetic data. Biochem J, 2001, 358: 437-445
  • Snyman SJ, Huckett BI, Botha FC and Watt MP: A comparison of direct and indirect somatic morphogenesis for the production of transgenic sugarcane (Saccharum spp. hybrids). Acta Hort, 2001, 560: 105-108

 

2000

  • Bindon KA and Botha FC: Tissue disks as an experimental system for metabolic flux analysis in the sugarcane culm. S A J Bot, 2000, 66: 260-264
  • Botha FC and Black K.G: Sucrose phosphate synthase and sucrose synthase activity during maturation of internodal tissue in sugarcane. Australian J Plant Physiol, 2000, 27: 81-85
  • Carson DL and Botha FC: Preliminary analysis of Expressed Sequence Tags for sugarcane. Crop Sci, 2000, 40: 1769-1779
  • Carson DL, Huckett BI and Botha FC: Patterns of gene expression in sugarcane monitored using cDNA macroarrays. Proceedings of the South African Sugar Technologists' Association, 2000, 74: 181-183
  • Groenewald J-H, Hiten F and Botha FC: The introduction of an inverted repeat to the 5' untranslated leader sequence of a transgene strongly inhibits gene expression. Plant Cell Reports, 2000, 19: 1098-1101
  • Meyer GM, Keeping MB, Watt DA, Cassim TN, Botha FC and Huckett BI: A wild-type insecticidal gene from a bacterium is poorly expressed in sugarcane: an overview. Proceedings of the South African Sugar Technologists' Association, 2000, 74: 184-185
  • Rose S and Botha FC: Distribution patterns of neutral invertase and sugar content in sugarcane internodal tissues. Plant Physiol Biochem, 2000, 38: 819-824.
  • Snyman SJ, Watt MP, Huckett BI and Botha FC: Direct somatic embryogenesis for rapid, cost effective production of transgenic sugarcane (Saccharum spp hybrids). Proceedings of the South African Sugar Technologists' Association, 2000, 74: 186-187