Equipment database DNA Sequencer

Covaris S2 Focused ultrasonicator

Model: S2

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: Deliver controlled, focused acoustic energy to a range of samples types

Purpose: DNA and RNA shearing, cell lysis

Eppendorf Refrigerated Centrifuge

Model: 5804R

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: Refrigerated centrifugation for samples in plate format

Purpose: Separation of particles from solution according to size, shape, density and viscosity

Agilent BioAnalyzer

Model: 2100

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: Chip-based capillary electrophoresis of DNA, RNA and protein

Purpose: Fragment analysis, quantification, and quality control of DNA, RNA and protein

Class II Biological Safety Cabinet

Model: LabGard ES NU-437S

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: Protection against contamination of samples, environment, and personnel

Purpose: Reduce potential for exposure of product/ personnel to airborne biological agents

Tecan liquid handler

Model: Freedom evo

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: Liquid handling with robotic functionality for general purpose pipetting

Purpose: Laboratory process automation

Qubit 4 Fluorometer

Model: 4.0

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: Detection of fluorescent dyes that bind to specific target molecules

Purpose: Precision fluorometric quantification of DNA, RNA and protein

PerkinElmer LabChip Nucleic Acid Analyzer

Model: GX Touch 24

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: Automated microfluidic capillary electrophoresis

Purpose: Quantification and sizing of DNA and RNA

MiniAmp Thermal cycler

Model: MiniAmp

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: Programmed temperature regulation in for denaturation and reannealing of DNA

Purpose: Amplify DNA and RNA

Agilent 2200 TapeStation

Model: 2200

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: Automated electrophoresis platform

Purpose: Sizing, quantification and quality control of RNA and DNA for downstream workflows

GeneAmp 9700 PCR system

Model: 9700

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: Programmed temperature regulation in for denaturation and reannealing of DNA

Purpose: Amplify DNA and RNA

Sage Science Pippen Prep

Model: Pippen Prep

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: Automated agarose gel electrophoresis

Purpose: Size selection of nucleic acids for sequencing workflows

NanoDrop spectrophotometer

Model: ND-1000

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: Spectrophotometric microvolume quantitation of nucleic acids and proteins

Purpose: Quantify and assess purity of DNA, RNA and proteins

Step One Plus Real-time PCR system

Model: Step One Plus

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: Amplification and fluorometric real-time quantification of double stranded DNA

Purpose: Closed system genotyping and quantification of amplifying DNA

Verity Thermal cycler

Model: Verity

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: Programmed temperature regulation in for denaturation and reannealing of DNA

Purpose: Amplify DNA and RNA

3730xl DNA Analyser

Model: 3730xl

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: High-throughput capillary electrophoresis with fluorescence detection

Purpose: Sequencing and fragment analysis including microsatellites, AFLP, SNP analysis

3500xl Genetic Analyzer

Model: 3500xl

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: Medium-throughput capillary electrophoresis with fluorescence detection

Purpose: Sequencing and fragment analysis including microsatellites, AFLP, SNP analysis

GeneTitan Multi-Channel instrument

Model: GeneTitan

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: Microarray processing

Purpose: Genotyping and expression studies

Ion Personal Genome Machine

Model: PGM

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: Massively parallel DNA fragment sequencing

Purpose: Targeted DNA and RNA sequencing, copy number analysis, small RNA sequencing, de novo microbial sequencing, genotyping by sequencing, ChIP sequencing

Ion GeneStudio S5 Prime

Model: Prime

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: Massively parallel DNA fragment sequencing

Purpose: Targeted DNA and RNA sequencing; copy number analysis, small RNA sequencing, de novo microbial sequencing, genotyping by sequencing, ChIP sequencing, exome and transcriptome sequencing

Proton Semiconductor sequencer

Model: Proton

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: Massively parallel DNA fragment sequencing

Purpose: Targeted DNA and RNA sequencing; copy number analysis, small RNA sequencing, de novo microbial sequencing, genotyping by sequencing, ChIP sequencing, exome and transcriptome sequencing

Ion S5 Semiconductor Sequencer

Model: S5

Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102

Function: Massively parallel DNA fragment sequencing

Purpose: Targeted DNA and RNA sequencing; copy number analysis, small RNA sequencing, de novo microbial sequencing, genotyping by sequencing, ChIP sequencing, exome and transcriptome sequencing

Ion Chef™ Instrument