Equipment database DNA Sequencer
Covaris S2 Focused ultrasonicator
Model: S2
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Deliver controlled, focused acoustic energy to a range of samples types
Purpose: DNA and RNA shearing, cell lysis
Eppendorf Refrigerated Centrifuge
Model: 5804R
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Refrigerated centrifugation for samples in plate format
Purpose: Separation of particles from solution according to size, shape, density and viscosity
Agilent BioAnalyzer
Model: 2100
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Chip-based capillary electrophoresis of DNA, RNA and protein
Purpose: Fragment analysis, quantification, and quality control of DNA, RNA and protein
Class II Biological Safety Cabinet
Model: LabGard ES NU-437S
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Protection against contamination of samples, environment, and personnel
Purpose: Reduce potential for exposure of product/ personnel to airborne biological agents
Tecan liquid handler
Model: Freedom evo
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Liquid handling with robotic functionality for general purpose pipetting
Purpose: Laboratory process automation
Qubit 4 Fluorometer
Model: 4.0
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Detection of fluorescent dyes that bind to specific target molecules
Purpose: Precision fluorometric quantification of DNA, RNA and protein
PerkinElmer LabChip Nucleic Acid Analyzer
Model: GX Touch 24
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Automated microfluidic capillary electrophoresis
Purpose: Quantification and sizing of DNA and RNA
MiniAmp Thermal cycler
Model: MiniAmp
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Programmed temperature regulation in for denaturation and reannealing of DNA
Purpose: Amplify DNA and RNA
Agilent 2200 TapeStation
Model: 2200
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Automated electrophoresis platform
Purpose: Sizing, quantification and quality control of RNA and DNA for downstream workflows
GeneAmp 9700 PCR system
Model: 9700
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Programmed temperature regulation in for denaturation and reannealing of DNA
Purpose: Amplify DNA and RNA
Sage Science Pippen Prep
Model: Pippen Prep
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Automated agarose gel electrophoresis
Purpose: Size selection of nucleic acids for sequencing workflows
NanoDrop spectrophotometer
Model: ND-1000
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Spectrophotometric microvolume quantitation of nucleic acids and proteins
Purpose: Quantify and assess purity of DNA, RNA and proteins
Step One Plus Real-time PCR system
Model: Step One Plus
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Amplification and fluorometric real-time quantification of double stranded DNA
Purpose: Closed system genotyping and quantification of amplifying DNA
Verity Thermal cycler
Model: Verity
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Programmed temperature regulation in for denaturation and reannealing of DNA
Purpose: Amplify DNA and RNA
3730xl DNA Analyser
Model: 3730xl
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: High-throughput capillary electrophoresis with fluorescence detection
Purpose: Sequencing and fragment analysis including microsatellites, AFLP, SNP analysis
3500xl Genetic Analyzer
Model: 3500xl
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Medium-throughput capillary electrophoresis with fluorescence detection
Purpose: Sequencing and fragment analysis including microsatellites, AFLP, SNP analysis
GeneTitan Multi-Channel instrument
Model: GeneTitan
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Microarray processing
Purpose: Genotyping and expression studies
Ion Personal Genome Machine
Model: PGM
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Massively parallel DNA fragment sequencing
Purpose: Targeted DNA and RNA sequencing, copy number analysis, small RNA sequencing, de novo microbial sequencing, genotyping by sequencing, ChIP sequencing
Ion GeneStudio S5 Prime
Model: Prime
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Massively parallel DNA fragment sequencing
Purpose: Targeted DNA and RNA sequencing; copy number analysis, small RNA sequencing, de novo microbial sequencing, genotyping by sequencing, ChIP sequencing, exome and transcriptome sequencing
Proton Semiconductor sequencer
Model: Proton
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Massively parallel DNA fragment sequencing
Purpose: Targeted DNA and RNA sequencing; copy number analysis, small RNA sequencing, de novo microbial sequencing, genotyping by sequencing, ChIP sequencing, exome and transcriptome sequencing
Ion S5 Semiconductor Sequencer
Model: S5
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Massively parallel DNA fragment sequencing
Purpose: Targeted DNA and RNA sequencing; copy number analysis, small RNA sequencing, de novo microbial sequencing, genotyping by sequencing, ChIP sequencing, exome and transcriptome sequencing
Ion Chef™ Instrument
Model: Chef
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Closed system, robotic liquid handler
Purpose: Automated library preparation, template preparation, and chip loading for sequencing of ion sphere particles on semiconductor sequencers
AB Library Builder
Model: Library builder
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Automation of library preparation or DNA extraction protocols
Purpose: Library preparation for massively parallel sequencing applications or DNA extraction
Nuaire Glacier -86 Ultra-Low Temperature Freezer
Model: Glacier
Location: Stellenbosch, Van der Bijl Street, JC Smuts building, Lab B102
Function: Ultralow freezer
Purpose: Preserve samples at ultralow (-80 Degrees) freezing ranges