Animal TB
Welcome to Stellenbosch University

Division of Molecular Biology & Human Genetics

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Animal TB

Alumni

Charlene Clarke completed her BScAgric degree at Stellenbosch University, returning to complete her MSc in Molecular Biology (graduated 2017).  Her thesis was titled "Investigation of temporal changes in immune responses to Mycobacterium bovis in cattle and African buffaloes (Syncerus caffer)".

 

 

 


Ross McFadyen completed his BSc (HonsMedSc) and MSc in Molecular Biology with the Animal TB Group in 2015.  His thesis was titled "Immunological tools for the characterization of the humoral immune response to Mycobacterium bovis infection in African buffaloes (Syncerus caffer)".

 

 

 


Ig Viljoen worked as a volunteer at the Wildlife Biological Resource Centre before doing a BSc in Zoology and Biochemistry and his BSc (Hons) and MSc in Conservation Biology at North-West University. His PhD project investigates the effects of M. bovis infection on the energy metabolism, immune and reproductive systems of lions.


 


 

Yessica Fitzermann graduated from the Animal TB Group with her MSc entitled: “Genomic characterisation of Mycobacterium mungi, the dassie bacillus, and Mycobacterium suricattae" in December 2017. This project investigated the relationship of these animal-adapted members of the M. tuberculosis complex and resulted in the development of a phylogenetic tree including new isolates from her study. Yessica has returned to her home in Germany but hopes to return to South Africa in the near future.


 


 

Manana Dlangalala completed her Honour's studies in Molecular Biology by writing a literature review entitled “Measurement of viral load in Lentivirus infections" and conducting research resulting in her thesis “Development of a reverse-transcription polymerase chain reaction assay for feline immunodeficiency virus of African lions (Panthera leo)". Manana is planning to continue her MSc at the University of Pretoria in 2018.


 


 

 

Roxanne Higgitt joined the Animal TB Research Group for her Honour's degree in 2015. During this year, she developed qPCR assays to detect upregulation of IFN-γ and CXCL genes in spotted hyenas exposed to Mycobacterium bovis. She continued with the group as a Master's candidate, during which she investigated M. bovis infection is another African carnivore, the African wild dog. During her degree, she developed an IFN-γ release assay (IGRA) to detect immune sensitisation to M. bovis in wild dogs. Using this assay, she estimated the IGRA prevalence of M. bovis in the Kruger National Park wild dog population and culture-confirmed M. bovis infection in this population. Currently, she is working as a Research Assistant for the Host-Pathogen Mycobactomics research group in the Department of Biomedical Sciences. 


​​Taime Sylvester joined the Department of Biomedical Sciences at Stellenbosch University in 2014 when she started her PhD in Molecular Biology, focusing on characterizing the immune response of African lions to Mycobacterium bovis infection. Before joining Stellenb​osch University she did her undergraduate training in Biomedical Technology at the Cape Peninsula University of Technology. Here she also completed her Masters in Biomedical Technology, focusing on evaluating the cardioprotection of kolaviron in ischemia-reperfusion. In 2018, Taime joined the TB Genomics research group as a post-doctoral fellow, where she aims to investigate the molecular epidemiology of tuberculosis in Namibia. 


Eduard Roos joined the Animal TB Research Group for his MSc in 2015. His MSc project looked at developing novel diagnostic assays for detecting warthogs with Mycobacterium bovis, the bacteria that causes TB in animals, infections. During 2016 he successfully upgraded his MSc project to a PhD, where he further investigated the humoral and cell-mediated immune response of warthogs in order to develop and evaluate a range of diagnostic assays for this species. He was also able to genetically characterise M. bovis isolates obtained from warthogs. These showed great diversity in some of the warthog populations and newer molecular techniques such as whole genome sequencing were able to distinguish between seemingly similar strains. Eduard completed his PhD in 2018 and obtained a position as a Postdoctoral Scientist at The Pirbright Institute. He will be developing and evaluating the utility of novel immune reagents for the Immunological Toolbox. The project aims to remove barriers to veterinary vaccine research. Orcid0000- 0002-3679-4995


Leeré Scott majored in Biochemistry and Microbiology for her BSc in Molecular Biology and Biotechnology at Stellenbosch University, where after she completed her BSc (Hons) degree with the Animal TB research group. Her Honours project focused on characterizing CXCL10 gene expression and IP-10 protein production in unstimulated whole blood from cows. For her MSc, she joined the TB Genomics research group and her project will be focusing on the investigation of specifically engineered Rv0678resistant associated variants, and their effect on the minimum inhibitory concentrations (MICs) of anti-TB drugs. Additionally, she will be investigating how heterogeneity in Rv0678 variants impact the MIC of a specific anti-TB drug, bedaquiline (BDQ). Results may provide insight into how multidrug resistance of Mycobacterium tuberculosis is influenced by Rv0678variants and how that may affect TB treatment outcomes and regimens. 


Sedzani Ndou completed her BSc (Hons) degree with the Animal TB research group in 2018. Her project focused on the investigation of RNA stabilization techniques in wildlife and domestic animal species. For her MSc, she joined the Immunology group and is under the supervision of Prof Novel Chegou.