Animal TB
Welcome to Stellenbosch University

​Division of Molecular Biology & Human Genetics​

Animal TB Research Group


  1. Gibson, K.M., Nguyen, B.N., Neumann, L.M., Miller, M., Buss, P., Daniels, S., Ahn, M.J., Crandall, K.A. and Pukazhenthi, B., 2019. Gut microbiome differences between wild and captive black rhinoceros–implications for rhino health. Scientific reports9(1), p.7570. 
  2. Edwards, K.L., Miller, M.A., Carlstead, K. and Brown, J.L., 2019. Relationships between housing and management factors and clinical health events in elephants in North American zoos. PloS One14(6), p.e0217774. 
  3. Byrne, A.W., Allen, A., O'Brien, D.J. and Miller, M.A., 2019. Bovine tuberculosis–international perspectives on epidemiology and management. Frontiers in Veterinary Science6, p.202. 
  4. Miller, M.A., Buss, P.E., Roos, E.O., Hausler, G., Dippenaar, A., Mitchell, E., Van Schalkwyk, O.L., Robbe- Austerman, S., Waters, W., Sikar-Gang, A. and Lyashchenko, K.P., 2019. Fatal tuberculosis in a free-ranging African elephant and one health implications of human pathogens in wildlife. Frontiers in Veterinary Science6
  5. Bernitz, N., Kerr, T.J., Goosen, W.J., Clarke, C., Higgitt, R., Roos, E.O., Cooper, D.V., Warren, R.M., van Helden, P.D., Parsons, S.D. and Miller, M.A., 2019. Parallel measurement of IFN-γ and IP-10 in QuantiFERON®- TB Gold (QFT) plasma improves the detection of Mycobacterium bovis infection in African buffaloes (Syncerus caffer). Preventive Veterinary Medicine169, p.104700. 
  6. Chileshe, J., Roos, E.O., Goosen, W.J., Buss, P., Hausler, G., Rossouw, L., Manemela, T., van Helden, P., Warren, R., Parsons, S.D. and Miller, M., 2019. An interferon-gamma release assay for the diagnosis of the Mycobacterium bovis infection in white rhinoceros (Ceratotherium simum). Veterinary Immunology and Immunopathology217, p.109931. 
  7. Kerr, T.J., de Waal, C.R., Buss, P.E., Hofmeyr, J., Lyashchenko, K.P. and Miller, M.A., 2019. Seroprevalence of Mycobacterium tuberculosis complex in free-ranging African elephants (Loxodonta africana) in Kruger National 
  8. Park, South Africa. Journal of Wildlife Diseases, 55(4), pp.923-927. Chileshe, J., Goosen, W.J., Buss, P.E., van Helden, P.D., Warren, R., Parsons, S.D. and Miller, M.A., 2019. A commercial ELISA for detection of interferon-gamma in white rhinoceros. Journal of Veterinary Diagnostic Investigation, p.1040638719843955. 
  9. Miller, M.A., Buss, P., Sylvester, T.T., Lyashchenko, K.P., deKlerk-Lorist, L.M., Bengis, R., Hofmeyr, M., Hofmeyr, J., Mathebula, N., Hausler, G. and van Helden, P., 2019. Mycobacterium bovis in free-ranging lions (Panthera leo)— evaluation of serological and tuberculin skin tests for detection of infection and disease. Journal of Zoo and Wildlife Medicine50(1), pp.7-15. 
  10. Pohlin, F., Buss, P., Miller, M., Steenkamp, G., Gleed, R., Poore, L., Boesch, J. and Zeiler, G., 2019. Etorphine- ketamine constant rate infusion for maintenance of anaesthesia in a compromised white rhinoceros (Ceratotherium simum). Case Reports in Veterinary Medicine2019
  11. Rosen, L.E., Olea-Popelka, F., Deem, S.L., Iszaz, R., Schmitt, D. and Miller, M., 2019. Survey of antituberculosis drug administration and adverse effects in elephants in North America. Journal of Zoo and Wildlife Medicine50(1), pp.23-32.
  12. Bernitz, N., Kerr, T.J., Goosen, W.J., Higgitt, R.L., de Waal, C., Clarke, C., Cooper, D.V., Warren, R.M. van Helden, P.D., Parsons, S.D.C. and Miller, M.A., 2019. Impact of Mycobacterium bovis-induced pathology on interpretation of QuantiFERON®-TB Gold assay results in African buffaloes (Syncerus caffer). Veterinary Immunology and Immunopathology,
  13. Viljoen, I.M., Sylvester, T.T., Parsons, S.D.C., Millar, R.P., van Helden, P.D. and Miller, M.A., 2019. Performance of the tuberculin skin test in Mycobacterium bovis-exposed and -unexposed African lions (Panthera leo). Journal of Wildlife Diseases, 55(3), pp. 537-543. 
  14. Roos, E.O., Scott, L.A., Ndou, S., Olea-Popelka, F., Buss, P.E., de Klerk-Lorist, L.-M., Warren, R.M., van Helden, P.D., Sylvester, T.T., Miller, M.A. and Parsons, S.D.C., 2019. Cytokine gene expression assay as a diagnostic tool for detection of Mycobacterium bovis infection in warthogs (Phacochoerus africanus). Scientific Reports 9:16525. doi:10.1038/s41598-019-53045-0 
  15. Neiffer, D., Buss, P., Hewlett, J., Hausler, G., Rossouw, L., Manamela, T., Grenus, B., Thulson, E., Olea-Popelka, F. and Miller, M., 2019. Evaluation of physiological parameters and effectiveness of an immobilization protocol using etorphine, azaperone, and butorphanol in free-ranging warthogs (Phacochoerus africanus). Frontiers in Veterinary Science, doi: 10.3389/fvets.2019.00402 
  16. Miller, M.A., Gortazar, C., Roos, E.O., Risalde, M.A., Johnathan-Lee, A., Sridhara, A.A. and Lyashchenko, K.P., 2019. Serological reactivity to MPB83 and CFP10/ESAT-6 antigens in three suid hosts of Mycobacterium bovis infection. Veterinary microbiology235, pp.285-288. 
  17. Love, D.M., Garner, M.M., Lyashchenko, K.P., Sikar-Gang, A., Bradway, D.S., Robbe-Austerman, S., Miller, M., and Ramer, J. Tuberculosis caused by Mycbacteriuym orygis in a greater one-horned rhinoceros (Rhinoceros unicornis): first report in the western hemisphere. Journal of Zoo and Wildlife Medicine, IN PRESS. 
  18. Bernitz, N., Kerr, T.J., de Waal, C., Cooper, D.V., Warren, R.M., van Helden, P.D., Parsons, S.D.C., and Miller, M.A. Test characteristics of assays to detect Mycobacterium bovis infection in high-prevalence African buffalo (Syncerus caffer) herds. Journal of Wildlife Diseases, IN PRESS. 
  19. Warren, R.M., Ismail, N., Chegou, N., Walzl, G., Kinnear, C., Goosen, W., Miller, M., Diacon, A. and van Helden, P.D. Tuberculosis in South Africa over the past 50 years: from bench to bedside. South African Medical Journal, IN PRESS. 
  20. Hewlett, J., Buss, P., Olea-Popelka, F., Koeppel, K., Neiffer, D., Hausler, G., Rossouw, L., Manamela, T., Stout, E. and Miller, M. Evaluation of a partially reversible immobilization protocol using medetomidine, butorphanol, zolazepam-tiletamine, and ketamine in free-ranging warthogs (Phacochoerus africanus) in Kruger National Park, South Africa. Journal of Zoo and Wildlife Medicine, IN PRESS. 
  21. Hooijberg, E.H., Cray, C., Steenkamp, G., Buss, P., Goddard, A. and Miller, M. Assessment of the acute phase response in healthy and injured southern white rhinoceros (Ceratotherium simum simum). Frontiers in Veterinary Science, IN PRESS. 
  22. Kerr, T.J., Gumbo, R., Goosen, W.J., Rogers, P., Last, R.D. and Miller, M.A. Novel detection techniques for Mycobacterium bovis infection in a cheetah (Acinonyx jubatus). Emerging Infectious Diseases, IN PRESS. 
  23. Krajewska, M., Didkowska, A., Sridhara, A., Elahi, R., Johnathan-Lee, A., Radulski, L., Lipiec, M., Anusz, K., Lyashchenko, K., Miller, M. and Waters, R. Transboundary tuberculosis: importation of alpacas infected with Mycobacterium bovis from the United Kingdom to Poland and potential for serodiagnostic assays detecting tuberculin skin test false-negative animals. Transboundary and Emerging Diseases, In revision. 
  24. Goosen, W.J., Kerr, T.J., Kleynhans, L., Warren, R.M., van Helden, P.D., Persing, D., Parsons, S.D.C., Buss, P. and Miller, M.A. The GeneXpert MTB/RIF Ultra assay detects Mycobacterium tuberculosis complex DNA in white rhinoceros (Ceratotherium simum) and African elephants (Loxodonta africana). In review. 
  25. Achkar, J.M., Adams, L.B., Dupnik, K.M., Hall-Stoodley, L., Kanipe, C., Kraigsley, A., Kurtz, S., Miller, M.A., Salvador, L., Larsen, M. and Robinson, R. The many hosts of mycobacteria: a conference report. In review. 
  26. Radcliffe, R.W., Jago, M., Morkel, P.V., Parry, S.A., Morkel, E., du Preez, P., Beytell, P., du Preez, J., Miller, M.A., Felippe, J. and Gleed, R.D. The pulmonary and metabolic effects of suspension by the feet compared with lateral recumbency in black rhinoceroses (Diceros bicornis) recently captured by aerial darting. In review. 
  27. Goosen, W.J., Kerr, T.J., Kleyhans, L., Buss, P., Warren, R.M., van Helden, P.D., Schroder, B., Parsons, S.D.C. and Miller, M.A. The VetMAXTM M. tuberculosis complex PCR Kit detects MTBC DNA in antemortem and postmortem samples from white rhinoceros (Ceratotherium simum), African elephants (Loxodonta africana) and African buffaloes (Syncerus caffer). In review. 

    Book Chapter 
    Buss, P. and Miller, M., 2019. Update on Field Anesthesia Protocols for Free-Ranging African Lions. In Fowler's Zoo and Wild Animal Medicine Current Therapy, Volume 9 (pp. 536-538). WB Saunders. ​