Institute for Plant Biotechnology
Welcome to Stellenbosch University

Recent Publications


  • Masoabi M, Lloyd J, Kossmann J and van der Vyver C.  Ethyl methanesulfonate mutagenesis and in vitro polyethylene glycol selection for drought tolerance in sugarcane (Saccharum sp.).  SugarTech, 2017, DOI: 10.1007/s12355-017-0524-8 
  • Ndimba RJ, Kruger J, Mehlo L, Barnabas A, Kossmann J and Ndimiba BK. A comparative study of selected physical and biochemical traits of wild-type and transgenic sorghum to reveal differences relevant to grain quality.  Frontiers in Plant Science, 2017, DOI: 10.3389/fpls.2017.00952
  • Strydom L, Jewell J, Meier MA and Lloyd J.  Analysis of genes involved in glycogen degradation in Escherichia coli.  FEMS Microbiology Letters, 2017, DOI: 10.1093/femsle/fnx016
  • Ndimba R, Cloete K, Mehlo L, Kossmann J, Mtshali CB, Pineda-Vargas C.  Using ICP and micro-PIXE to investigate possible differences in the mineral composition of genetically modified versus wild-type sorghum grain. Nuclear Instruments and Methods in Physics Research Section B Beam Interactions with Materials and Atoms, 2017, DOI10.1016/j.nimb.2017.03.004


  • Saner P, Philipson CD, Peters S, Keller F, Bigler L, Turnbull LA and Hector LA.  Growth rates and relative change in non-structural carbohydrates of dipterocarp seedlings in response to light acclimation.  Plant Ecology and Diversity, 2016, DOI: 10.1080/17550874.2016.1227385
  • Lloyd JR and Kotting O.  Starch biosynthesis and degradation in plants.  In: eLS. John Wiley & Sons, Ltd: Chichester. DOI: 10.1002/9780470015902.a0020124.pub2
  • Le Roux ML, Botha A-M and van der Vyver C. Somatic embryogenesis and cryopreservation of South African bread wheat (Triticum aestivum L.) genotypes. South African Journal of Botany, 2016, 106: 78-88
  • Thuynsma R, Kleinert A, Kossmann J, Valentine AJ and Hills PN. The effects of limiting phosphate on photosynthesis and growth of Lotus japonicas. South African Journal of Botany, 2016, 104: 244-248.
  • Rose L, Mouton M, Beukes I, Flett BC, van der Vyver C and Viljoen A. Multi-environment evaluation of maize inbred lines for resistance to Fusarium ear rot and fumonisins. Plant Disease, 2016,


  • Fly R, Lloyd JR, Krueger S, Fernie A, van der Merwe MJ. 2015. Improvements to define mitochondrial metabolomics using non-aqueous fractionation . In: Springer Protocols 1305; Plant Mitochondria: Methods and Protocols. Humana Press. Eds. Whelan, J & Murcha MW. pp197-210.  DOI 10.1007/978-1-4939-2639-8_15
  • Hector S, Willard K, Bauer R, Mulako I, Slabbert E, Kossmann J, George GM. Diverse exopolysaccharide producing bacteria isolated from milled sugarcane: Implications for cane spoilage and sucrose yield. PLOS ONE, 2015, 10 (12):E0145487. DOI:10.1371/journal.pone.0145487
  • Egert A, Eicher B, Keller F, Peters S.  Evidence for water deficit-induced mass increases of raffinose family oligosaccharides (RFOs) in the leaves of three Craterostigma resurrection plant species.  Frontiers in Physiology, 2015, 6: 206. DOI:10.3389/fphys.2015.00206
  • George GM, Ruckle ME, Lloyd JR. 2015. Virus-induced gene silencing as a scalable tool to study drought tolerance. In: Springer Protocols 1287; Plant Gene Silencing: Methods and Protocols. Humana Press. Eds. Mysore, KS & Muthappa, S. pp243-253. DOI 10.1007/978-1-4939-2453-0_18
  • Lloyd JR, Kossmann J: Transitory and storage starch metabolism: two sides of the same coin? Current Opinion in Biotechnology, 2015, 32: 143-148. DOI:10.1016/j.copbio.2014.11.026 


  • Grobbelaar MC, Makunga NP, Stander MA, Kossmann J, Hills PN: Effect of strigolactones and auxins on growth and metabolite content of Sutherlandia frutescens (L.) R. Br. microplants in vitro. Plant Cell, Tissue and Organ Culture, 2014, 117(3): 401-409
  • Kleinert A, Venter M, Kossmann J, Valentine A: The reallocation of carbon in P deficient lupins affects biological nitrogen fixation. Journal of Plant Physiology, 2014, 171(17): 1619-1624. DOI:10.1016/j.jplph.2014.07.0717
  • Christ B, Egert A, Sussenbacher I, Krautler B, Bartels D, Peters S, Hortensteiner S: Water deficit induces chlorophyll degradation via the 'PAO/phyllobilin' pathway in leaves of homoio- (Craterostigma pumilum) and poikilochlorophyllous (Xerophyta viscosa) resurrection plants. Plant, Cell and Environment, 2014, 37(11): 2521-2531


  • Egert A, Keller F, Peters S: Abiotic stress-induced accumulation of raffinose in Arabidopsis leaves is mediated by a single raffinose synthase (RS5, At5g40390). BMC Plant Biology, 2013, 13: 2118
  • Sonnewald U, Kossmann J: Starches – from current models to genetic engineering. Plant Biotechnology Journal, 2013, 11(2): 223-232
  • Van der Vyver C, Conradie T, Kossmann J, Lloyd J: In vitro selection of transgenic sugarcane callus utilizing a plant gene encoding a mutant form of acetolactate synthase.  In Vitro Cellular and Developmental Biology – Plant, 2013, 49 (2): 198-206


  • Bauer R, Hiten F, Crouch AM, Kossmann J, Burger BV: Acrolein dimer as a marker for direct detection of acrolein in wine. South African Journal of Enology and Viticulture, 2012, 33(1): 72-79
  • Egert A, Peters S, Guyot C, Stieger B, Keller F: An Arabidopsis T-DNA Insertion Mutant for galactokinase (AtGALK, At3g06580) hyperaccumulates free galactose and is insensitive to exogenous galactose. Plant Cell Physiology, 2012, 53(5): 921–929
  • Albrecht CF, Stander MA, Grobbelaar MC, Colling J, Kossmann J, Hills PN, Makunga NP: LC–MS-based metabolomics assists with quality assessment and traceability of wild and cultivated plants of Sutherlandia frutescens (Fabaceae). South African Journal of Botany, 2012, 82: 33-45
  • Cronje C, George GM, Fernie AR, Bekker J, Kossmann J, Bauer R: Manipulation of L-ascorbic acid biosynthesis pathways in Solanum lycopersicum: elevated GDP-mannose pyrophosphorylase activity enhances L-ascorbate levels in red fruit. Planta, 2012, 235(3): 553-564
  • Bauer R, Basson CE, Bekker J, Eduardo I, Rohwer JM, Uys L, van Wyk JH, Kossmann J:  Reuteran and levan as carbohydrate sinks in transgenic sugarcane.  Planta, 2012 236: 1803-1815 
  • Pillay P, Kibido T, du Plessis M, van der Vyver C, Beyene G, Vorster BJ, Kunert K, Schluter U: Use of transgenic Oryzacystatin-I expressing plants enhances recombinant protein production.  Applied Biochem and Biotech, 2012, 168 (6): 1608-1620
  • George GM, Bauer R, Blennow A, Kossmann J, Lloyd JR: Virus induced multiple gene silencing to study redundant metabolic pathways in plants: Silencing the starch degradation pathway in Nicotiana benthamiana. Biotechnology Journal, 2012, 7(7): 884-890
  • Figlan S, Kossmann J, Makunga N: Seed germination behaviour, micropropagation and Agrobacterium-mediated transformation of Salvia runcinata (L.f.): implications for conservation and cultivation. South African Journal of Botany, 2012, 79:18
  • Gouws LM, Botes E, Wiese AJ, Trenkamp S, Torres-Jerez I, Tang Y, Hills PN, Usadel B, Lloyd JR, Fernie AR, Kossmann J, van der Merwe MJ: The plant growth promoting substance, lumichrome, mimics starch and ethane-associated symbiotic responses in Lotus and tomato roots. Frontiers of Plant Science, 2012, 3:120 


  • Burger BV, Viviers MZ, le Roux NJ, Morris J, Bekker JPI, le Roux M: Olfactory cue mediated neonatal recognition in sheep, Ovis aries. Journal of Chemical Ecology, 2011, 10:1150-1163
  • Cronje C, George GM, Fernie AR, Bekker J, Kossmann J, Bauer R: Manipulation of L-ascorbic acid biosynthesis pathways in Solanum lycopersicum: elevated GDP-mannose pyrophosphorylase activity enhances L-ascorbate levels in red fruit. Planta, 2011, 235: 553-564
  • George GM, van der Merwe M, Nunes-Nesi A, Bauer R, Fernie AR, Kossmann J, Lloyd JR: Virus-induced gene silencing of plastidial soluble inorganic pyrophosphatase impairs essential leaf anabolic pathways and reduces drought stress tolerance in Nicotiana benthamiana. Plant Physiology, 2011, 154: 55-66
  • van der Vyver C, Vorster BJ, Kunert K, Cullis CA: Analysis of radiation-induced genome alterations in Vigna unguiculata. Research and Reports in Biology, 2011, 2: 89-99