Online Training: 27 June - 6 July 2022
Application process and cost of participation
Applications open on 16 May and the online application process is open until
6 June 2022.
Applications are OPEN: Click here to register for the online training.
All researchers, postgraduate students and university and industry technical staff may apply.
The training is free.
Contact cafadmin@sun.ac.za for more information.
2022 Workshops:
1. Basic introduction to X-ray computed tomography & 3D imaging
Presenter: Muofhe Tshibalanganda
Pre-recorded, will be available for questions after the video.
The workshop will cover the basics of X-ray Micro-CT and Nano-CT.
Course content will include:
· Specific examples
· How the method works
· What kind of information can be found
· Advantages of X-ray 3D imaging
Systems will be demonstrated and prepared data will be used for demonstrating 3D data analysis. This will cover basic 3D visualization, image segmentation, defect analysis, part to part comparison and many more. The course focuses on new users and is ideal for researchers and students new to the field of non-destructive "X-ray microscopy".
___
2. Water Isotope Analysis by Laser Spectroscopy
Presenter: Dr Jared van Rooyen
Pre-recorded
"The purpose of this course is to provide comprehensive hands-on training in the installation, operation and maintenance of laser spectrometry for the analysis of stable isotopes in water samples and the use of the LIMS for Lasers software.
The course comprises lectures covering theoretical concepts on the measurement of environmental stable isotopes using laser spectrometry, followed by demonstrations and practical exercises including the setup of the laser analyser, sample preparation, as well as a brief overview of isotope data postprocessing.
___
3. Basic principals of Ion
Chromatography
Presenter: Dr Janine Colling
Pre-recorded
This workshop will focus on an introduction to the theory of Ion chromatography principles and present an overview of the instrumentation and sample analysis.
___
4. Introduction to
Hyperspectral imaging and chemometrics
Presenter: Dr Janine Colling
Pre-recorded
During this workshop, you will receive two videos to follow. The first video will provide an overview of the theory and principles of this technique to enable a better understanding of how NIR spectroscopy and hyperspectral imaging works. It will describe typical applications which make use of this instrument and technique. The second video will focus on an introduction to multivariate data analysis which can be used to explore trends in data and to develop qualitative and quantitative models.
___
5. Introduction to Total organic carbon (TOC) analysis
Volante Moonsamy
Pre-recorded
This workshop will cover the:
- Theory and background principles, which are important to understand TOC analysis
- Discuss the general principles of how the instrumentation operates and how total organic carbon (TOC), total inorganic carbon (TIC) and total carbon (TC) analysis takes place
- Include an overview of sample preparation guidelines
___
6. Sample preparation for elemental analysis in rock, sediment, environmental & biological samples for measurement by XRF, ICP & CHNS
Presenters: Riana Rossouw, Mareli Grobbelaar & Charney Small
Pre-recorded
Sample preparation is a critical step towards accurate elemental analysis. This presentation explains different types of preparation selected according to the specific analytical technique appropriate to your sample types and the outcome required.
___
7. Introduction to XRF Spectrometry
Presenter: Mareli Grobbelaar
Pre-recorded
XRF spectrometry is used for major and minor element quantification on a range of solid sample types. This presentation explains the principles of the technique.
___
8. Laser Ablation ICP-MS in Earth and Environmental Science
Presenter: Riana Rossouw
Pre-recorded
This presentation explains the principle and application of Laser ablation ICP-MS to measure minor and trace elements in bulk sediments, minerals or selected types of biological samples.
___
9. Major and trace element quantification of liquid and solid samples by ICP analysis
Presenters: Riana Rossouw & Charney Small
Pre-recorded
Water, food, beverages, soils and biological material is analysed for major, minor and trace element composition using ICP measurement after appropriate sample processing. Understand the principles of the technique. It is advised to first watch our Sample Preparation presentation.
___
10. An introduction to Serial Block-Face (SBF) Scanning Electron Microscopy (SEM)
Presenter: Jurgen Kriel
Live
Serial Block-Face (SBF) Scanning Electron Microscopy (SEM) is a powerful imaging modality that allows for 3D acquisition SEM samples at high resolution. This is of great value for biological research, as cells and tissues can be studied in their native 3D morphology which allows for a deeper understanding of cellular phenotypes associated with certain pathologies. When combined with other fluorescent microscopy techniques, SBF-SEM provides immense value for the localization and distribution of proteins, bacteria or viruses within tissues which can provide novel insights into certain disease states. This workshop will serve as an introduction to SBF-SEM and explain the workflow involved in sample preparation and image acquisition. Due to the large amount of data generated by this approach, an overview of data handling and image processing will also be given to introduce researchers to basic principles of machine learning in the era of 'Big Data'.
___
11. Introduction to biological sample preparation for SEM
Presenter: Madelaine Frazenburg
Pre-recorded
Biological sample preparation is essential in scanning electron microscopy (SEM). Flawed sample preparations can undermine the quality of results and lead to false conclusions. Thus, the aim of this talk is to equip researchers and students with the essential knowledge required to prepare samples for SEM investigations in life sciences. To obtain acceptable SEM images with good ultrastructural preservation requires careful application of the SEM sample preparation method.
___
12. Basic principles for Imaging Electron Microscopy
Presenter: Madelaine Frazenburg
Pre-recorded
The talk offers an overview of the technical application and principles of using a scanning electron microscope (SEM) to produce high-resolution electron images by choosing the correct and appropriate settings and detectors. The advantages, signal detection from the different detectors get discussed in detail. The effect of on how to perfect and image using the different tools available on the SEM.
___
13. Introducing to biological sample preparation for STEM
Presenter: Dr Alicia Botes
Pre-recorded
This talk presents an overview of the components and principles of STEM. You will also learn how-to optimize STEM for specific samples. We talk about the advantages of STEM with the availability of various types of STEM techniques, different detectors and a variety of adjustments in order to suit specific samples and analysis requirements. We also mention any limitations of STEM and how to overcome it using these tools.
___
14. Basic principles for Imaging Scanning Transmission Electron
Presenter: Dr Alicia Botes
Pre-recorded
In this talk, we outline the history of STEM and go on to explain the sample preparation required for STEM and how it influences the imaging quality during the analysis. We focus mainly on sample preparation for biological samples since the preparation of these types of samples is commonly the most complex. The discussion includes various staining agents, thin sectioning and the various grid types available for use in STEM and TEM.
15. Introduction to Flow Cytometry
Presenter: Dr Dalene de Swardt
___
16. Optimization in Flow Cytometry
Presenter: Dr Dalene de Swardt
___
17. Introduction to Spectral Flow Cytometry
Presenter: Dr Dalene de Swardt
___
18. Introduction to Imaging Flow Cytometry
Presenter: Dr Dalene de Swardt
___
19. Correlative Light and Electron Microscopy
Presenters: Lize Engelbrecht and Jurgen Kriel
The workshop on correlative light and electron microscopy will show the participant how two microscopy modalities, confocal/super-resolution microscopy and scanning electron microscopy, can be used on the same sample to exploit more information from the sample than would have been possible with any of the two modalities separately. The benefits of this technique and the different workflows to achieve correlation will be discussed, but also the inherent challenges of the technique. We will also introduce an open source software package we use for ultimate correlation.
___
20. Super-resolution
Microscopy
Presenter: Lize Engelbrecht
In this workshop, the participants will be introduced to the latest developments in the field of super-resolution microscopy techniques. The ability of microscopes to resolve detail in fluorescence images at sub-resolution limits is becoming more and more accessible to biological researchers. In the workshop we will explain in more detail also the specific aspects of SR SIM and SMLM, two of the super-resolution techniques we can perform here in Stellenbosch.
___
21. Mass Spectrometry of Small Molecules: GCMS
Presenter: Lucky Mokwena
We look forward to your participation!
