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Fluorescence Microscopy Training

June 2018 Training Initiative Workshops - click here

15-16 March 2018  Confocal Microscopy Course – max 6 persons

(R1800 per person for internal users, R2000 person for external academic people)

This workshop is aimed to introduce the participant to the principals of fluorescence microscopy and its applications. This includes multicolour experiments, 3D imaging, and introduction to super-resolution platforms. Approximately 40% of the course is spent on theory and lectures, while 60% is spent on the confocal microscope itself where participants will get some hands-on experience. The course is recommended for all participants who want to apply confocal microscopy to their work and all users who want to become independent users are expected to complete this course.

 

19-20 March 2018  Introduction to Flow Cytometry Course – max 6 persons

(R1800 per person for internal users, R2000 person for external academic people)

The Introduction to Flow Cytometry is a  two-day basic course aimed at beginners, and encompasses both lectures and hands on training. The course will cover lectures on the principle of the method, instrument setup, quality control, choosing fluorochromes and dyes, as well as compensation and spill over. Participants will also receive hands on experience in the  setup and analysis of a 4 colour experiment as well. The course is recommended for all participants who want to apply flow cytometry to their work and all users who want to become independent users are expected to complete this course.

 

22 March 2018   Cell sorting on the new BD FACS Melody – max 4 persons

(R900 per person for internal users, R1000 person for external academic people)

Students interested in sorting samples for further analysis, can attend our one-day cell sorting workshop on the new BD FACSMelody. The course is 80% hands on training and aimed at users with some prior flow cytometry experience who understand the principles of the method. The course will cover instrument setup for sorting and quality control, as well as training on tube sorting, single cell sorting in plate format and single cell sorting onto microscope slides, or participants who have attended our basic flow cytometry workshop. The training will also cover optimisation of the sample preparation for sorting and further culture.

 

9-10 April 2018  PALM/STORM workshop – max 4 persons

(Cost to be confirmed – please indicate interest at this stage)

A workshop on PALM/STORM super-resolution microscopy will be presented jointly by myself and an international visitor from Zeiss. The first morning will be an introductory presentation on PALM/STORM and acquisition of some sample material. The afternoon and second day would be spent on analysing and processing data that had been acquired already and will be led by Zeiss. This aspect has proven to be most challenging and data quality depends heavily on good post-acquisition processing. This workshop is recommended for those who might be using this super-resolution platform for their current or future projects.

 

12 April 2018   Flow cytometry data analysis workshop – FlowJo – max 10 persons

(R600 per person for internal users, R700 per person for external academic people)

With this one-day course we aim to teach users basic data analysis of their flow cytometric data in FlowJo, a popular third party software package for analysis. We will cover data input, compensation, gating, creating tables  and layouts for visual and data output. This course is aimed at allowing users to become more independent with their data analysis.