Stellenbosch University
Welcome to Stellenbosch University
Proteomics Workshop
Start: 26/03/2019, 08:00
End: 28/03/2019, 17:00
Contact:Dr Mare Vlok -
Location: Tygerberg

​26-28 March 2019

Mass Spectrometry Based Proteomics

Presenter: Dr Maré Vlok

Duration: 3 Days

After this 3-day workshop the student will know the basic principles of mass spectrometry, be able to prepare biological samples for mass spectrometry-based analysis and be able to use global databases for mass spectrometry-based data in order to identify proteins. Finally, the student will be able to recognise the value of mass spectrometry for their own research.

Course will comprise of the following:

  • Theoretical component: basic principles of mass spectrometry and proteomics, overview of various proteomics and mass spectrometry based applications.
  • Tutorials: Design of proteomics and mass spectrometry based experiments.
  • Practicals: Preparation of biological samples for mass spectrometry with the specific aim of proteomics based applications. Therefore, samples will be used from gels and will be cleaned, trypsin digested and finally prepared for identification by mass spectrometry. Data obtained from the practical will then be used for database searches in order to teach the student the basic principles that are important for database searches.
  • Discussions: After each component time is allocated for questions and answers, the course is also ended with an in depth discussion from the students and how they would apply mass spectrometry within their own projects.

Day 1:
Lecture: Introduction to Proteomics, sample extraction and preparation for digest
Practical: Prepare samples for in-gel and in-solution digest
Discussion: Any questions on the day?

Day 2:
Practical: Extraction and clean-up of peptides
Practical: Preparation of MS system
Lecture: Chromatographic separation and enrichment techniques
Lecture: Mass spectrometry
Lecture: Quantitative proteomics
Discussion: Questions?

Day 3:
Practical: Preparing database search
Practical: Investigating data
Lecture: Data analysis and statistics
Discussion: Students feedback on how they can apply Mass spectrometry based proteomics in their projects.

Click here to apply