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Study about the resistance to Roundup (Glyphosate) uses DNA Sequencing
Author: A Vorster
Published: 24/11/2020

​The herbicide, Roundup, has been used over the past 45 years in the Western Cape.  As a result of this constant, high selection pressure, resistance to Roundup (Glyphosate) has developed in a range of weeds.  Sparse genome data for these low-priority genomes, complicates investigations into the genetic determinants of resistance.  

Mr Vhuthu Ndou, a PhD candidate in the Department of Agronomy at Stellenbosch University, is studying the mechanism(s) of Glyphosate resistance in a selected Plantago lanceolata biotype.  RNA extraction, cDNA synthesis, PCR assay design and sequencing of a specific candidate gene, through the DNA sequencing unit, has supported his investigations towards identifying molecular mechanisms for Glyphosate resistance.